Application
Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 5 μg/mL.Application
Immunofluorescence of CIDE-A in Human Brain cells with CIDE-A antibody at 20 μg/mL.Application
Western blot analysis of CIDE-A in human brain tissue lysate in the absence (A) or presence (B) of peptide (2085P) with CIDE-A antibody at 1:2000 dilution.| Product Name | CIDE-A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CIDE-A Antibody: CIDE-A, Cell death activator CIDE-A, Cell death-inducing DFFA-like effector A |
| Product description | CIDE-A Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors. Cell death signals are transduced by DD-, DED-, or CARD-containing molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase DFF40/CAD, which is chaperoned and inhibited by DFF45/ICAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is expressed in many tissues.1) Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184 2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50 |
| Immunogen | CIDE-A antibody was raised against a 18 amino acid peptide near the carboxy terminus of human CIDE-A. The immunogen is located within the last 50 amino acids of CIDE-A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CIDE-A antibody can be used for detection of CIDE-A by Western blot at 2 μg/mL. An approximately 23 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CIDEA |
|---|---|
| Alternative Names | CIDE-A Antibody: CIDE-A Cell death activator CIDE-A Cell death-inducing DFFA-like effector A |
| Molecular Weight(MW) | 23 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | It has no cross activity to CIDE-B. |
Application
Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 5 μg/mL.
Application
Immunofluorescence of CIDE-A in Human Brain cells with CIDE-A antibody at 20 μg/mL.
Application
Western blot analysis of CIDE-A in human brain tissue lysate in the absence (A) or presence (B) of peptide (2085P) with CIDE-A antibody at 1:2000 dilution.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | CIDE-A antibody can be used for detection of CIDE-A by Western blot at 2 μg/mL. An approximately 23 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CIDE-A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CIDE-A Antibody is supplied in PBS containing 0.02% sodium azide. |
