Application
Immunohistochemistry of CIDE-A in mouse heart tissue with CIDE-A antibody at 5 μg/mL.Application
Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 2.5 μg/ml.Application
Immunofluorescence of CIDE-A in Mouse Heart cells with CIDE-A antibody at 20 ug/mL.Application
Western blot analysis of CIDE-A in mouse heart tissue lysate with CIDE-A antibody at 1:500 dilution.| Product Name | CIDE-A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CIDE-A Antibody: CIDE-A, Cell death activator CIDE-A, Cell death-inducing DFFA-like effector A |
| Product description | CIDE-A Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-A is expressed in many tissues.1) Inohara N, Koseki T, Chen S, et al. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33 |
| Immunogen | CIDE-A antibody was raised against an 18 amino acid peptide near the carboxy terminus of mouse CIDE-A. The immunogen is located within the last 50 amino acids of CIDE-A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CIDE-A antibody can be used for detection of CIDE-A by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CIDEA |
|---|---|
| Alternative Names | CIDE-A Antibody: CIDE-A Cell death activator CIDE-A Cell death-inducing DFFA-like effector A |
| Molecular Weight(MW) | 25 kDa |
| Tissue Specificity | It has no cross activity to CIDE-B. |
Application
Immunohistochemistry of CIDE-A in mouse heart tissue with CIDE-A antibody at 5 μg/mL.
Application
Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 2.5 μg/ml.
Application
Immunofluorescence of CIDE-A in Mouse Heart cells with CIDE-A antibody at 20 ug/mL.
Application
Western blot analysis of CIDE-A in mouse heart tissue lysate with CIDE-A antibody at 1:500 dilution.| Positive Control | 1) Cat. No. 1401 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | CIDE-A antibody can be used for detection of CIDE-A by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CIDE-A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CIDE-A Antibody is supplied in PBS containing 0.02% sodium azide. |
