Application
Western blot analysis of CIDE-B in mouse liver tissue lysate with CIDE-B antibody at 1:500 dilution.| Product Name | CIDE-B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CIDE-B Antibody: CIDE-B, AI790179, 1110030C18Rik, Cell death activator CIDE-B, Cell death-inducing DFFA-like effector B |
| Product description | CIDE-B Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-B induces apoptosis, which is inhibited by DFF45. CIDE-B is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-B is expressed mainly in liver and at lower levels in spleen, kidney, peripheral blood lymphocytes, and bone marrow.1) Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33 |
| Immunogen | CIDE-B antibody was raised against a 16 amino acid peptide near the carboxy terminus of murine CIDE-B. The immunogen is located within the last 50 amino acids of CIDE-B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CIDE-B antibody can be used for detection of CIDE-B by Western blot 0.5 μg/mL. An approximately 25 kDa band can be detected.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Cideb |
|---|---|
| Alternative Names | CIDE-B Antibody: CIDE-B AI790179 1110030C18Rik Cell death activator CIDE-B Cell death-inducing DFFA-like effector B |
| Molecular Weight(MW) | 25 kDa |
| Tissue Specificity | It has no cross activity to CIDE-A. |
Application
Western blot analysis of CIDE-B in mouse liver tissue lysate with CIDE-B antibody at 1:500 dilution.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
|---|---|
| Application Notes | CIDE-B antibody can be used for detection of CIDE-B by Western blot 0.5 μg/mL. An approximately 25 kDa band can be detected.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CIDE-B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CIDE-B Antibody is supplied in PBS containing 0.02% sodium azide. |
