Application
Immunohistochemistry of CIDE-B in human small intestine tissue with CIDE-B antibody at 5 μg/mL.Application
Immunofluorescence of CIDE-B in Human Small Intestine cells with CIDE-B antibody at 20 μg/mL.Application
Western blot analysis of CIDE-B in mouse small intestine tissue lysate with CIDE-B antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | CIDE-B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CIDE-B Antibody: CIDE-B, AI790179, 1110030C18Rik, Cell death activator CIDE-B, Cell death-inducing DFFA-like effector B |
| Product description | CIDE-B Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-B induces apoptosis, which is inhibited by DFF45. CIDE-B is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-B is expressed mainly in liver and at lower levels in spleen, kidney, peripheral blood lymphocytes and bone marrow.1) Inohara N, Koseki T, Chen S,et al. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J. 1998; 17:2526-33. |
| Immunogen | CIDE-B antibody was raised against a peptide corresponding to 14 amino acids near the center of human CIDE-B. The immunogen is located within amino acids 100 - 150 of CIDE-B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CIDE-B antibody can be used for detection of CIDE-B by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Cideb |
|---|---|
| Alternative Names | CIDE-B Antibody: CIDE-B AI790179 1110030C18Rik Cell death activator CIDE-B Cell death-inducing DFFA-like effector B |
| Sequence Similarities | 3) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | CIDE-B antibody has no cross activity to CIDE-A. |
Application
Immunohistochemistry of CIDE-B in human small intestine tissue with CIDE-B antibody at 5 μg/mL.
Application
Immunofluorescence of CIDE-B in Human Small Intestine cells with CIDE-B antibody at 20 μg/mL.
Application
Western blot analysis of CIDE-B in mouse small intestine tissue lysate with CIDE-B antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1408 - Mouse Small Intestine Tissue Lysate |
|---|---|
| Application Notes | CIDE-B antibody can be used for detection of CIDE-B by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CIDE-B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CIDE-B Antibody is supplied in PBS containing 0.02% sodium azide. |
