Application
Immunohistochemical analysis of paraffin - embedded human normal liver tissue (top) and human colon carcinoma (bottom) showing membrane location using CK1 antibody with DAB staining.Application
Western blot analysis using CK1 monoclonal antibody against truncated CK1 recombinant protein.| Product Name | CK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CK1, CKI-alpha, CK1a, CKIa, HLCDGP1, PRO2975, HEL-S-77p |
| Product description | CK1 (also designated Cytokeratin 1 or KRT1 ),with 644-amino acid protein (about 70 kDa), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells.CK1 consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK1 is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.1) Ricardo G. Espinola, Robin A. Pixley, Berhane Ghebrehiwet. Blood (ASH Annual Meeting Abstracts), Nov 2005; 106: 2666. |
| Immunogen | Ni-NTA purified truncated recombinant CK1 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CSNK1A1 |
|---|---|
| Alternative Names | CK1 CKI-alpha CK1a CKIa HLCDGP1 PRO2975 HEL-S-77p |
Application
Immunohistochemical analysis of paraffin - embedded human normal liver tissue (top) and human colon carcinoma (bottom) showing membrane location using CK1 antibody with DAB staining.
Application
Western blot analysis using CK1 monoclonal antibody against truncated CK1 recombinant protein.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CK1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
