Application
Western blot analysis of CKIP1 in human lung tissue lysate with CKIP1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CKIP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CKIP1 Antibody: OC120, CKIP-1, CKIP1, OC120, HQ0024c, Pleckstrin homology domain-containing family O member 1, C-Jun-binding protein, PH domain-containing family O member 1 |
| Product description | CKIP1 Antibody: CKIP1 was identified through a yeast-two hybrid screening as a protein that would interact with only one of the two catalytic subunits of the casein kinase 2 complex. CKIP1 is a pleckstrin homology domain-containing protein localized within the nucleus and at the plasma membrane that interacts with CK2alpha but not CK2alpha', and is thought to play a role in targeting CK2alpha to a particular cellular location. CKIP1 has been implicated in muscle differentiation and the regulation of cell morphology and actin cytoskeleton. CKIP1 can also interact with other proteins such as ATM, an upstream kinase of p53, and recruit the nuclear ATM to the plasma membrane, suggesting CKIP1 may regulate ATM function through re-localizing ATM. CKIP1 can also form a complex with the kinase AKT, leading to a down-regulation of PI3K/AKT signaling and suppression of tumor growth in vivo.1) Bosc DG, Graham KC, Sauliner RB, et al. Identification and characterization of CKIP-1, a novel pleckstrin homology domain-containing protein that interacts with protein kinase CK2. J. Biol. Chem.2000; 275:14295-306. |
| Immunogen | CKIP1 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human CKIP1. The immunogen is located within the last 50 amino acids of CKIP1. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CKIP1 antibody can be used for detection of CKIP1 by Western blot at 1:500-2000: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Predicted species reactivity based on immunogen sequence: Bovine: (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLEKHO1 |
|---|---|
| Alternative Names | CKIP1 Antibody: OC120 CKIP-1 CKIP1 OC120 HQ0024c Pleckstrin homology domain-containing family O member 1 C-Jun-binding protein PH domain-containing family O member 1 |
Application
Western blot analysis of CKIP1 in human lung tissue lysate with CKIP1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | CKIP1 antibody can be used for detection of CKIP1 by Western blot at 1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | CKIP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CKIP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
