Application
Western blot analysis of CLASP2 Antibody (Y1019) in mouse spleen tissue lysates (35ug/lane)Application
Flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CLASP using rabbit polyclonal CLASP Antibody (Y1019) using 293 cell lysates transfected with the ACADL gene (Lane 1) and with the GFP-CLASP (Lane 2).| Product Name | CLASP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLIP-associating protein 2, Cytoplasmic linker-associated protein 2, Protein Orbit homolog 2, hOrbit2, CLASP2, KIAA0627 |
| Product description | CLASP2 is microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.1) Inkster,B., et.al., Neuroimage (2010) In press. |
| Immunogen | This CLASP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 998-1026 amino acids from human CLASP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLASP2 |
|---|---|
| Alternative Names | CLIP-associating protein 2 Cytoplasmic linker-associated protein 2 Protein Orbit homolog 2 hOrbit2 CLASP2 KIAA0627 |
| Molecular Weight(MW) | 141 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis of CLASP2 Antibody (Y1019) in mouse spleen tissue lysates (35ug/lane)
Application
Flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CLASP using rabbit polyclonal CLASP Antibody (Y1019) using 293 cell lysates transfected with the ACADL gene (Lane 1) and with the GFP-CLASP (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
