Application
Western blot analysis of lysates from Hela cell line and human placenta tissue lysate(from left to right), using COL4A1 Antibody at 1:1000 at each lane.Application
Fluorescent image of U251 cells stained with COL4A1 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).Application
Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: DU145 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | COL4A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Collagen alpha-1(IV) chain, Arresten, COL4A1 |
| Product description | COL4A1 is the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.1) de Vries,L.S., Ann. Neurol. 65 (1), 12-18 (2009) |
| Immunogen | This COL4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-54 amino acids from the N-terminal region of human COL4A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:25: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL4A1 |
|---|---|
| Alternative Names | Collagen alpha-1(IV) chain Arresten COL4A1 |
| Molecular Weight(MW) | 161 kDa |
Application
Western blot analysis of lysates from Hela cell line and human placenta tissue lysate(from left to right), using COL4A1 Antibody at 1:1000 at each lane.
Application
Fluorescent image of U251 cells stained with COL4A1 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Application
Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: DU145 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:25: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
