Application
Western blot analysis in mouse bladder tissue lysates (35ug/lane).Application
COL5A1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in U251 cell line lysates (35ug/lane).| Product Name | COL5A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Collagen alpha-1(V) chain, COL5A1 |
| Product description | COL5A1 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. COL5A1 product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq].1) Vitart, V., et al. Hum. Mol. Genet. 19(21):4304-4311(2010) |
| Immunogen | This COL5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-82 amino acids from the N-terminal region of human COL5A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL5A1 |
|---|---|
| Alternative Names | Collagen alpha-1(V) chain COL5A1 |
| Molecular Weight(MW) | 184 kDa |
Application
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Application
COL5A1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in U251 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
