WB
WB analysis of HeLa using COL5A1 Monoclonal antibody. HeLa cells were subjected to SDS PAGE followed by western blot with COL5A1 Monoclonal antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.WB
WB analysis of mouse colon using COL5A1 Monoclonal antibody. mouse colon tissue were subjected to SDS PAGE followed by western blot with COL5A1 Monoclonal antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.WB
WB analysis using COL5A1 Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with COL5A1 Monoclonal antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
IHC staining of human breast cancer using COL5A1 Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using COL5A1 Monoclonal antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
IF Staining of U2OS using COL5A1 Monoclonal antibody. Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Collagen Type V antibody at dilution of 1:800 and 488-Conjugated Goat Anti-Mouse IgG(H+L).| Product Name | COL5A1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | native protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000-1:50000 IHC:1:500-1:2000 ICC/IF:1:400-1:1600 |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | COL5A1 |
|---|---|
| Gene Synonyms | EDSC FMDMF EDSCL1 |
| Gene Full Name | collagen type V alpha 1 chain |
| Gene Summary | This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] |
| Alternative Names | 2C2C2 Collagen alpha 1(V) chain Collagen alpha-1(V) chain Collagen Type V |
| Molecular Weight(MW) | 184 kDa(Observed band size:240-250 kDa) |
| Cellular Localization | Extracellular matrix,Secreted. |
WB
WB analysis of HeLa using COL5A1 Monoclonal antibody. HeLa cells were subjected to SDS PAGE followed by western blot with COL5A1 Monoclonal antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB
WB analysis of mouse colon using COL5A1 Monoclonal antibody. mouse colon tissue were subjected to SDS PAGE followed by western blot with COL5A1 Monoclonal antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB
WB analysis using COL5A1 Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with COL5A1 Monoclonal antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human breast cancer using COL5A1 Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using COL5A1 Monoclonal antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
IF Staining of U2OS using COL5A1 Monoclonal antibody. Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Collagen Type V antibody at dilution of 1:800 and 488-Conjugated Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:5000-1:50000 IHC:1:500-1:2000 ICC/IF:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
