WB
Western blot analysis of lysates from HeLa, and HUVEC, and HepG2 cells, using Collagen V alpha1 Antibody. The lane on the right is blocked with the synthesized peptide.IHC
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Collagen V alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.ICC/IF
Immunofluorescence analysis of HeLa cells, using Collagen V alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.| Product Name | COL5A1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen V alpha1. AA range:301-350. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL5A1 |
|---|---|
| Gene Synonyms | EDSC FMDMF EDSCL1 |
| Gene Full Name | collagen type V alpha 1 chain |
| Gene Summary | This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] |
| Alternative Names | COL5A1 Collagen alpha-1 V chain |
| Molecular Weight(MW) | 200 kDa |
| Cellular Localization | Secreted , extracellular space , extracellular matrix . |
WB
Western blot analysis of lysates from HeLa, and HUVEC, and HepG2 cells, using Collagen V alpha1 Antibody. The lane on the right is blocked with the synthesized peptide.
IHC
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Collagen V alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF
Immunofluorescence analysis of HeLa cells, using Collagen V alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
