Application
Immunohistochemistry staining of Collagen IV in kidney tissue using Collagen IV Antibody.| Product Name | Collagen IV Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ICH, HANAC, POREN1, arresten |
| Product description | Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with type I, II, III, V, or VI collagens. |
| Immunogen | Collagen IV antibody was raised against amino acids from the C-Terminus of Collagen Type IV from human and bovine placenta (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| Collagen IV antibody can be used in immunohistochemistry starting at 20 μg/mL.: |
|
| Species Reactivity | BovineHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL4A1 |
|---|---|
| Alternative Names | ICH HANAC POREN1 arresten |
Application
Immunohistochemistry staining of Collagen IV in kidney tissue using Collagen IV Antibody.| Application Notes | Collagen IV antibody can be used in immunohistochemistry starting at 20 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store Collagen IV antibody at 4 ˚C prior to opening. This product is stable at 4 ˚C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20 ˚C or below. |
| Storage Buffer | 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide. |
