COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP. COPS2 is also innvolved in early stage of neuronal differentiation via its interaction with NIF3L1.1) Moehren, U., (2004) (er) Nucleic Acids Res. 32 (10), 2995-3004.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human COPS2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
COPS2 antibody can be used for detection of COPS2 by ELISA at 1:312500. COPS2 antibody can be used for detection of COPS2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HEK293T cells at 2.5 ug/ml.
Application
Antibody used in IHC on Human Skin.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
COPS2 antibody can be used for detection of COPS2 by ELISA at 1:312500. COPS2 antibody can be used for detection of COPS2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store COPS2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt