Application
Flow cytometric analysis of MCF-7cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of COX5A Antibody in Hela cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with COX5A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | COX5A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cytochrome c oxidase subunit 5A, mitochondrial, Cytochrome c oxidase polypeptide Va, COX5A |
| Product description | This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.1) Colinge J., et.al., Submitted (OCT-2008) to UniProtKB. |
| Immunogen | This COX5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-73 amino acids from the Central region of human COX5A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COX5A |
|---|---|
| Alternative Names | Cytochrome c oxidase subunit 5A mitochondrial Cytochrome c oxidase polypeptide Va COX5A |
| Molecular Weight(MW) | 17 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of MCF-7cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of COX5A Antibody in Hela cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with COX5A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
