CPSF6 is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitement of other processing factors. The cleavage factor complex is composed of four polypeptides.The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitement of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.1) Rual, J.F., (2005) Nature 437 (7062), 1173-1178.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CPSF6.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
CPSF6 antibody can be used for detection of CPSF6 by ELISA at 1:1562500. CPSF6 antibody can be used for detection of CPSF6 by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Jurkat cells at 0.3125 ug/ml.
Application
Antibody used in IHC on Human Muscle at 4.0-8.0 ug/ml.
Application
Antibody used in IF on SKOV3 at 4 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
CPSF6 antibody can be used for detection of CPSF6 by ELISA at 1:1562500. CPSF6 antibody can be used for detection of CPSF6 by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CPSF6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt