CREBZF strongly activates transcription when bound to HCFC1. CREBZF suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. It also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. It is able to down-regulate expression of some cellular genes in CREBZF-expressing cells.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CREBZF.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CREBZF antibody can be used for detection of CREBZF by ELISA at 1:1562500. CREBZF antibody can be used for detection of CREBZF by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human CCRT-CEM cells at 5.0 ug/ml.
Positive Control
1) CCRT-CEM Cell Lysate
Application Notes
CREBZF antibody can be used for detection of CREBZF by ELISA at 1:1562500. CREBZF antibody can be used for detection of CREBZF by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CREBZF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt