Application
Western blot analysis of ZHANGFEI in K562 cell lysate with ZHANGFEI antibody at 1 μg/mL.Application
Immunocytochemistry of ZHANGFEI in K562 cells with ZHANGFEI antibody at 10 μg/mL.| Product Name | ZHANGFEI Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZHANGFEI Antibody: ZF, SMILE, ZF, Host cell factor-binding transcription factor Zhangfei, HCF-binding transcription factor Zhangfei |
| Product description | ZHANGFEI Antibody: ZHANGFEI, also known as ZF, is a basic region-leucine zipper transcriptional factor that has been implicated in herpes virus infection cycle and related cellular processes. ZHANGFEI protein has a negatively charged active N-terminus, a leucine zipper of six heptad leucine repeats separated by a conserved six amino acid spacer, a basic domain and a bZIP region. ZHANGFEI strongly activates transcription and suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1 dependent manner. It also suppresses the HCFC1 dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell.1) Hogan MR, Cockram GP and Lu R. Cooperative interaction of Zhangfei and ATF4 in transactivation of the cyclic AMP response element. FEBS Lett. 2006; 580:58-62. |
| Immunogen | ZHANGFEI antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human ZHANGFEI. The immunogen is located within amino acids 180 - 230 of ZHANGFEI. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| ZHANGFEI antibody can be used for detection of ZHANGFEI by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CREBZF |
|---|---|
| Alternative Names | ZHANGFEI Antibody: ZF SMILE ZF Host cell factor-binding transcription factor Zhangfei HCF-binding transcription factor Zhangfei |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Entrez Gene | 58487 |
|---|---|
| Protein Accession | NP_001034707 |
Application
Western blot analysis of ZHANGFEI in K562 cell lysate with ZHANGFEI antibody at 1 μg/mL.
Application
Immunocytochemistry of ZHANGFEI in K562 cells with ZHANGFEI antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | ZHANGFEI antibody can be used for detection of ZHANGFEI by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZHANGFEI antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZHANGFEI Antibody is supplied in PBS containing 0.02% sodium azide. |
