Application
Immunofluorescence of CRH in human brain tissue with CRH antibody at 20 μg/mL.Application
Immunohistochemistry of CRH in human brain tissue with CRH antibody at 5 μg/mL.Application
Western blot analysis of CRH in human placenta tissue lysate with CRH antibody at (A) 1 and (B) 2 μg/ml.| Product Name | CRH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Corticotropin-releasing hormone (CRH) is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress (1). In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta where it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturitio (2). Marked reduction in this protein has been observed in association with Alzheimer disease (3).1) Maras PM and Baram TZ. Sculpting the hippocampus from within: stress, spines, and CRH. Trends Neurosci. 2012; 315-24. |
| Immunogen | CRH antibody was raised against a 16 amino acid peptide near the amino terminus of human CRH. The immunogen is located within amino acids 30 - 80 of CRH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CRH antibody can be used for detection of CRH by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. | |
| Species Reactivity | HumanRatMousePig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRH |
|---|---|
| Alternative Names | CRH Antibody: CRF Corticoliberin Corticotropin-releasing factor CRF |
| Molecular Weight(MW) | Predicted: 22 kDa Observed: 29 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | CRH antibody is human specific. |
Application
Immunofluorescence of CRH in human brain tissue with CRH antibody at 20 μg/mL.
Application
Immunohistochemistry of CRH in human brain tissue with CRH antibody at 5 μg/mL.
Application
Western blot analysis of CRH in human placenta tissue lysate with CRH antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1309 - Human Placenta Tissue Lysate |
|---|---|
| Application Notes | CRH antibody can be used for detection of CRH by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | CRH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CRH antibody is supplied in PBS containing 0.02% sodium azide. |
