WB
Various lysates were subjected to SDS PAGE followed by western blot with CRH/CRF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using CRH/CRF antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CRH/CRF antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Product Name | CRH/CRF Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CRH protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC:1:400-1:1600 IF-P:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.6mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRH |
|---|---|
| Gene Synonyms | CRF CRH1 |
| Gene Full Name | corticotropin releasing hormone |
| Gene Summary | This gene encodes a member of the corticotropin-releasing factor family. The encoded preproprotein is proteolytically processed to generate the mature neuropeptide hormone. In response to stress, this hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus, binds to corticotropin releasing hormone receptors and stimulates the release of adrenocorticotropic hormone from the pituitary gland. Marked reduction in this protein has been observed in association with Alzheimer's disease. Autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes, and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Nov 2015] |
| Alternative Names | CRH Corticoliberin Corticotropin releasing factor Corticotropin-releasing factor Corticotropin-releasing hormone |
| Molecular Weight(MW) | 27 kDa |
| Cellular Localization | Secreted. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with CRH/CRF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using CRH/CRF antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CRH/CRF antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Application Notes | WB:1:500-1:2000 IHC:1:400-1:1600 IF-P:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
