Application
Immunohistochemistry of CRTH2 in human heart tissue with CRTH2 antibody at 2.5 μg/mL.Application
Immunofluorescence of CRTH2 in Human Heart tissue with CRTH2 antibody at 20 μg/mL.Application
Western blot analysis of CRTH2 in Jurkat cell lysate with CRTH2 antibody at 1 μg/mL in (A) the absence and (B) presence of blocking peptide.| Product Name | CRTH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CRTH2 Antibody: The chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) is a recently identified receptor for the prostaglandin D2 (PGD2) in addition to the classic prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo.1) Nagata K, Hirai K, Tanaka K, et al. CRTH2, an orphan receptor of T-helper-2-cells, is expressed on basophils and eosinophils and responds to mast cell-derived factor(s). FEBS Lett. 1999; 459:195-9. |
| Immunogen | CRTH2 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human CRTH2. The immunogen is located within the first 50 amino acids of CRTH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CRTH2 antibody can be used for detection of CRTH2 by Western blot a 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPR44 |
|---|---|
| Alternative Names | CRTH2 Antibody: DP2 DL1R CD294 CRTH2 GPR44 Prostaglandin D2 receptor 2 Chemoattractant receptor-homologous molecule expressed on Th2 cells |
| Molecular Weight(MW) | Predicted: 43, 55 kDa Observed: 60 kDa |
| Sequence Similarities | 3) Cat. No. 10-501 - Human Heart Tissue Slide |
| Tissue Specificity | At least two different isoforms of CRTH2 are known to exist; this antibody will detect both isoforms. |
Application
Immunohistochemistry of CRTH2 in human heart tissue with CRTH2 antibody at 2.5 μg/mL.
Application
Immunofluorescence of CRTH2 in Human Heart tissue with CRTH2 antibody at 20 μg/mL.
Application
Western blot analysis of CRTH2 in Jurkat cell lysate with CRTH2 antibody at 1 μg/mL in (A) the absence and (B) presence of blocking peptide.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | CRTH2 antibody can be used for detection of CRTH2 by Western blot a 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | CRTH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CRTH2 Antibody is supplied in PBS containing 0.02% sodium azide. |
