Application
Immunofluorescence of CRTH2 in Human Heart cells with CRTH2 antibody at 20 μg/mL.Application
Immunohistochemistry of CRTH2 in human heart tissue with CRTH2 antibody at 2.5 μg/mL.Application
Western blot analysis of CRTH2 in human heart tissue lysate with CRTH2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CRTH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CRTH2 Antibody: The chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) is a recently identified receptor for the prostaglandin D2 (PGD2) in addition to the classic prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. At least two different isoforms of CRTH2 are known to exist.1) Nagata K, Hirai K, Tanaka K, et al. CRTH2, an orphan receptor of T-helper-2-cells, is expressed on basophils and eosinophils and responds to mast cell-derived factor(s). FEBS Lett. 1999; 459:195-9. |
| Immunogen | CRTH2 antibody was raised against a 15 amino acid synthetic peptide from near the center of human CRTH2. The immunogen is located within amino acids 220 - 270 of CRTH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CRTH2 antibody can be used for detection of CRTH2 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPR44 |
|---|---|
| Alternative Names | CRTH2 Antibody: DP2 DL1R CD294 CRTH2 GPR44 Prostaglandin D2 receptor 2 Chemoattractant receptor-homologous molecule expressed on Th2 cells |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
Application
Immunofluorescence of CRTH2 in Human Heart cells with CRTH2 antibody at 20 μg/mL.
Application
Immunohistochemistry of CRTH2 in human heart tissue with CRTH2 antibody at 2.5 μg/mL.
Application
Western blot analysis of CRTH2 in human heart tissue lysate with CRTH2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | CRTH2 antibody can be used for detection of CRTH2 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | CRTH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CRTH2 Antibody is supplied in PBS containing 0.02% sodium azide. |
