Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of CSF1R Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of lysate from U-937 cell line, using CSF1R Antibody at 1:1000.| Product Name | CSF1R Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CSF1R is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases.1) Guey,L.T., et.al., Eur. Urol. 57 (2), 283-292 (2010) |
| Immunogen | This CSF1R antibody is generated from rabbits immunized with CSF1R recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CSF1R |
|---|---|
| Alternative Names | Macrophage colony-stimulating factor 1 receptor CSF-1 receptor CSF-1-R CSF-1R M-CSF-R Proto-oncogene c-Fms CD115 CSF1R FMS |
| Molecular Weight(MW) | 108 kDa |
Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of CSF1R Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of lysate from U-937 cell line, using CSF1R Antibody at 1:1000.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
