Application
Immunohistochemical analysis of paraffin-embedded human brain, using CSFR (Phospho-Tyr561) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HepG2 cells treated with PMA using CSFR (Phospho-Tyr561) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | CSFR (phospho Tyr561) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development.1) Hampe A., Oncogene Res. 4:9-17(1989). |
| Immunogen | CSFR (phospho Tyr561) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 561 (N-S-Y (p)-T-F) derived from Human CSFR . |
| Modification | p-Tyr561 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CSF1R |
|---|---|
| Alternative Names | CD115 CSF-1-R CSFMR FMS M-CSFR |
| Molecular Weight(MW) | 130 kDa |
| Tissue Specificity | The antibody detects endogenous levels of CSFR only when phosphorylated at tyrosine 561. |
| Entrez Gene | 1436 |
|---|---|
| Protein Accession | NP_001275634.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain, using CSFR (Phospho-Tyr561) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HepG2 cells treated with PMA using CSFR (Phospho-Tyr561) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
