Application
Immunohistochemistry of CTHRC1 in human small intestine tissue with CTHRC1 antibody at 5 μg/mL.Application
Immunofluorescence of CTHRC1 in human small intestine tissue with CTHRC1 antibody at 20 μg/mL.Application
Western blot analysis of CTHRC1 in rat small intestine tissue lysate with CTHRC1 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | CTHRC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The Collagen triple helix repeat-containing protein (CTHRC1) is a glycosylated, secreted protein that is transiently expressed in the arterial wall in response to injury where it is thought to contribute to vascular remodeling by inhibiting collagen expression and deposition and promoting cell migration (1). CTHRC1 is highly expressed multiple human cancers, including pancreatic cancer where it plays a significant role in the progression and metastasis of the disease by regulating migration and adhesion of tumors cells (2). CTHRC1 overexpression can also be used as an independent prognostic marker in gastric cancer (3). Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma (4).1) Pyagay P, Heroult M, Wang Q, et al. Collagen triple helix repeat containing 1, a novel secreted protein in injured and diseased arteries, inhibits collagen expression and promotes cell migration. Circ. Res. 2005; 96:261-8. |
| Immunogen | CTHRC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human CTHRC1. The immunogen is located within the first 50 amino acids of CTHRC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CTHRC1 antibody can be used for detection of CTHRC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTHRC1 |
|---|---|
| Alternative Names | Collagen triple helix repeat-containing protein 1 |
| Molecular Weight(MW) | Predicted: 27 kDa Observed: 27 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | CTHRC1 antibody is human, mouse and rat reactive. At least two isoforms of CTHRC1 are known to exist; this antibody will detect only the larger isoform. |
Application
Immunohistochemistry of CTHRC1 in human small intestine tissue with CTHRC1 antibody at 5 μg/mL.
Application
Immunofluorescence of CTHRC1 in human small intestine tissue with CTHRC1 antibody at 20 μg/mL.
Application
Western blot analysis of CTHRC1 in rat small intestine tissue lysate with CTHRC1 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1468 - Rat Small Intestine Tissue Lysate |
|---|---|
| Application Notes | CTHRC1 antibody can be used for detection of CTHRC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | CTHRC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CTHRC1 antibody is supplied in PBS containing 0.02% sodium azide. |
