Application
Western blot analysis of CUEDC2 in HeLa cell lysate with CUEDC2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunocytochemistry of CUEDC2 in HeLa cells with CUEDC2 antibody at 5 μg/mL.| Product Name | CUEDC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CUEDC2 Antibody: The CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) domain is an evolutionarily conserved, ~40 amino acid monoubiquitin-binding domain that mediates intramolecular monoubiquitylation. CUE domains are present in eukaryotic proteins that are involved in ubiquitination and protein trafficking pathways and may be required for ubiquitination of the proteins in which they are found. CUEDC2 (CUE domain-containing protein 2) was found through a yeast two-hybrid screening as a protein that interacts with the progesterone receptor (PR) and promotes progesterone-induced PR degradation by the ubiquitin-proteasome pathway. CUEDC2 also decreases the sumoylation of PR. CUEDC2 has also been found to interact with IKK-alpha and IKK-beta and decrease the activation of NF-κB by decreasing the activation of IKK.1) Shih SC, Prag G, Francis SA, et al. A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J. 2003; 22:1273-81. |
| Immunogen | CUEDC2 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human CUEDC2. The immunogen is located within the last 50 amino acids of CUEDC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| CUEDC2 antibody can be used for detection of CUEDC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CUEDC2 |
|---|---|
| Alternative Names | CUEDC2 Antibody: C10orf66 bA18I14.5 C10orf66 HOYS6 CUE domain-containing protein 2 |
| Molecular Weight(MW) | Predicted: 32 kDa Observed: 34 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | CUEDC2 antibody will not cross-react with CUEDC1. |
Application
Western blot analysis of CUEDC2 in HeLa cell lysate with CUEDC2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunocytochemistry of CUEDC2 in HeLa cells with CUEDC2 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | CUEDC2 antibody can be used for detection of CUEDC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | CUEDC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CUEDC2 Antibody is supplied in PBS containing 0.02% sodium azide. |
