Application
Western blot analysis of CX3CL1 in C2C12 cell lysate with CX3CL1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | CX3CL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CX3CL1 Antibody: Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-α in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.1) Bajetto A, Bonavia R, Barbero S, et al. Chemokines and their receptors in the central nervous system. Front. Neuroendocrinol. 2001; 22:147-84. |
| Immunogen | CX3CL1 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human CX3CL1. The immunogen is located within amino acids 20 - 70 of CX3CL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| CX3CL1 antibody can be used for detection of CX3CL1 by Western blot at 0.5 - 2 μg/mL dilution. CX3CL1 is ofen observed migrating at 80 - 100 kDa in SDS-PAGE, presumably due to post-translational modification. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CX3CL1 |
|---|---|
| Alternative Names | CX3CL1 Antibody: NTN NTT CXC3 CXC3C SCYD1 ABCD-3 C3Xkine fractalkine neurotactin FKN A-152E5.2 Fractalkine C-X3-C motif chemokine 1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (79%) |
Application
Western blot analysis of CX3CL1 in C2C12 cell lysate with CX3CL1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Application Notes | CX3CL1 antibody can be used for detection of CX3CL1 by Western blot at 0.5 - 2 μg/mL dilution. CX3CL1 is ofen observed migrating at 80 - 100 kDa in SDS-PAGE, presumably due to post-translational modification. |
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| Form | Liquid |
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| Storage Instructions | CX3CL1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CX3CL1 Antibody is supplied in PBS containing 0.02% sodium azide. |
