WB
Western blot analysis of CX3CL1 on different lysates with Rabbit anti-CX3CL1 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; Low expression: skeletal muscle. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: CX3CL1 Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature. The molecular weight is consistent with the references.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Cortex. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: CX3CL1 Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Cortex. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: CX3CL1 Recombinant Rabbit Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IF-F
Application: Immunofluorescence (IHC-Fr). Species: Mouse. Tissue: Brain. Sample: Frozen section. Antigen retrieval: Not required. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: CX3CL1 Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.IF-F
Application: Immunofluorescence (IHC-Fr). Species: Rat. Tissue: Brain. Sample: Frozen section. Antigen retrieval: Not required. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: CX3CL1 Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.IP
CX3CL1 was immunoprecipitated in 0.2 mg mouse brain tissue lysate with CX3CL1 Recombinant Rabbit Monoclonal Antibodyat 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using CX3CL1 Recombinant Rabbit Monoclonal Antibodyat 1/5,000 dilution. Alpaca anti-Rabbit IgG Fc, Recombinant VHH monoclonal Antibody at 1/50,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain cell lysate (input). Lane 2: CX3CL1 Recombinant Rabbit Monoclonal AntibodyIP in mouse brain cell lysate. Lane 3: Rabbit IgG instead of CX3CL1 Recombinant Rabbit Monoclonal Antibodyin mouse brain cell lysate. Exposure time: 1 minute 18 seconds. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary dilution: CX3CL1 Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, 2 hours at room temperature. The molecular weight is consistent with the references.| Product Name | CX3CL1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse CX3CL1 aa 1-357. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCIPWB |
| WB:1:5000 IHC:1:200-1:1000 IF-F:1:500 IP:1-2μg/sample |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CX3CL1 |
|---|---|
| Gene Synonyms | NTN NTT CXC3 CXC3C SCYD1 ABCD-3 C3Xkine fractalkine neurotactin |
| Gene Full Name | C-X3-C motif chemokine ligand 1 |
| Gene Summary | This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections. [provided by RefSeq, Sep 2017] |
| Alternative Names | Fractalkine FK ABCD-3 C-X3-C motif chemokine 1 CX3C membrane-anchored chemokine Neurotactin Small-inducible cytokine D1 Processed fractalkine Cx3cl1 Cx3c Fkn Scyd1 |
| Molecular Weight(MW) | 42 kDa(Observed band size:75-100 kDa) |
| Cellular Localization | Cell membrane; Secreted. |
WB
Western blot analysis of CX3CL1 on different lysates with Rabbit anti-CX3CL1 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; Low expression: skeletal muscle. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: CX3CL1 Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature. The molecular weight is consistent with the references.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Cortex. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: CX3CL1 Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Cortex. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: CX3CL1 Recombinant Rabbit Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IF-F
Application: Immunofluorescence (IHC-Fr). Species: Mouse. Tissue: Brain. Sample: Frozen section. Antigen retrieval: Not required. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: CX3CL1 Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.
IF-F
Application: Immunofluorescence (IHC-Fr). Species: Rat. Tissue: Brain. Sample: Frozen section. Antigen retrieval: Not required. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: CX3CL1 Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.
IP
CX3CL1 was immunoprecipitated in 0.2 mg mouse brain tissue lysate with CX3CL1 Recombinant Rabbit Monoclonal Antibodyat 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using CX3CL1 Recombinant Rabbit Monoclonal Antibodyat 1/5,000 dilution. Alpaca anti-Rabbit IgG Fc, Recombinant VHH monoclonal Antibody at 1/50,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain cell lysate (input). Lane 2: CX3CL1 Recombinant Rabbit Monoclonal AntibodyIP in mouse brain cell lysate. Lane 3: Rabbit IgG instead of CX3CL1 Recombinant Rabbit Monoclonal Antibodyin mouse brain cell lysate. Exposure time: 1 minute 18 seconds. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary dilution: CX3CL1 Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, 2 hours at room temperature. The molecular weight is consistent with the references.| Application Notes | WB:1:5000 IHC:1:200-1:1000 IF-F:1:500 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
