WB
Western blot analysis of lysates from Mouse brain using CX3CL1 Rabbit pAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60 s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse brain tissue using CX3CL1 Rabbit pAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse intestine tissue using CX3CL1 Rabbit pAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse lung tissue using CX3CL1 Rabbit pAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC stainingIF-P
Immunofluorescence analysis of Mouse brain tissue using CX3CL1 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.| Product Name | CX3CL1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:5000 IHC:1:100-1:500 IF-P:1:100-1:300 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Cx3cl1 |
|---|---|
| Gene Synonyms | FK CX3C Cxc3 Scyd1 ABCD-3 D8Bwg0439e |
| Gene Full Name | C-X3-C motif chemokine ligand 1 |
| Gene Summary | Enables CX3C chemokine receptor binding activity. Acts upstream of or within several processes, including leukocyte chemotaxis; negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; and positive regulation of transforming growth factor beta1 production. Located in neuron projection; neuronal cell body; and plasma membrane. Is expressed in several structures, including brain; genitourinary system; gut; nose; and respiratory system. Human ortholog(s) of this gene implicated in Cor pulmonale and common cold. Orthologous to human CX3CL1 (C-X3-C motif chemokine ligand 1). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | NTN NTT CXC3 CXC3C SCYD1 ABCD-3 C3Xkine fractalkine neurotactin |
| Molecular Weight(MW) | 42 kDa(Observed MW 100 kDa) |
| Cellular Localization | Cell membrane, Secreted, Single-pass type I membrane protein. |
WB
Western blot analysis of lysates from Mouse brain using CX3CL1 Rabbit pAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60 s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse brain tissue using CX3CL1 Rabbit pAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse intestine tissue using CX3CL1 Rabbit pAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse lung tissue using CX3CL1 Rabbit pAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining
IF-P
Immunofluorescence analysis of Mouse brain tissue using CX3CL1 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.| Application Notes | WB:1:1000-1:5000 IHC:1:100-1:500 IF-P:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
