Application
Immunofluorescence of CX3CR1 in THP-1 cells with CX3CR1 antibody at 20 μg/mL.Application
Immunohistochemistry of CX3CR1 in human spleen cells with CX3CR1 antibody at 10 μg/mL.Application
Western blot analysis of CX3CR1 in THP-1 cell lysate with CX3CR1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | CX3CR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CX3CR1 Antibody: CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was initially identified as the receptor for the transmembrane molecule fractalkine, and renamed CX3CR1. CX3CR1 is known to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.1) Raport CJ, Schweickart VL, Eddy RL JR, et al. The orphan G-protein-coupled receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues. Gene 1995; 163:295-9 |
| Immunogen | CX3CR1 antibody was raised against a peptide corresponding to amino acids in a extracellular loop of human CX3CR1. The immunogen is located within amino acids 160 - 210 of CX3CR1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CX3CR1 antibody can be used for detection of CX3CR1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CX3CR1 |
|---|---|
| Alternative Names | CX3CR1 Antibody: V28 CCRL1 GPR13 CMKDR1 GPRV28 CMKBRL1 CX3C chemokine receptor 1 Beta chemokine receptor-like 1 C-X3-C CKR-1 |
| Molecular Weight(MW) | Predicted: 39, 43 kDa Observed: 50 kDa |
| Sequence Similarities | 3) Cat. No. 17-008 - THP-1 Cell Slide |
| Tissue Specificity | At least two isoforms of CX3CR1 are known to exist. |
Application
Immunofluorescence of CX3CR1 in THP-1 cells with CX3CR1 antibody at 20 μg/mL.
Application
Immunohistochemistry of CX3CR1 in human spleen cells with CX3CR1 antibody at 10 μg/mL.
Application
Western blot analysis of CX3CR1 in THP-1 cell lysate with CX3CR1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Application Notes | CX3CR1 antibody can be used for detection of CX3CR1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
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| Form | Liquid |
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| Storage Instructions | CX3CR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CX3CR1 Antibody is supplied in PBS containing 0.02% sodium azide. |
