Application
Immunocytochemistry of CXCR4 in HeLa cells with CXCR4 antibody at 2 μg/mL.Application
Flow cytometry analysis of HeLa cells using CXCR4 antibody at 0.1 μg/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.Application
Immunofluorescence of CXCR4 in HeLa cells with CXCR4 antibody at 20 μg/mL.Application
Western blot analysis of CXCR4 in HeLa whole cell lysate with CXCR4 antibody at 0.5 μg/mL.| Product Name | CXCR4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CXCR4 Antibody: Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites.1) Dimitrov DS. Cell 1997;91:721-730 |
| Immunogen | CXCR4 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human CXCR4. The immunogen is located within the first 50 amino acids of CXCR4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICCIFIPWB |
| CXCR4 antibody can be used for Western blot at 1 - 2 μg/mL dilution and for immunoprecipitation and immunocytochemistry at 10 μg/mL. Immunofluorescence starting at 20 μg/mL. Flow Cytometry at 0.1 μg/ml | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCR4 |
|---|---|
| Alternative Names | CXCR4 Antibody: FB22 HM89 LAP3 LCR1 NPYR WHIM CD184 LESTR NPY3R NPYRL HSY3RR NPYY3R D2S201E |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | CXCR4 Antibody is predicted to not cross-react with other CXCR familiy members. |
Application
Immunocytochemistry of CXCR4 in HeLa cells with CXCR4 antibody at 2 μg/mL.
Application
Flow cytometry analysis of HeLa cells using CXCR4 antibody at 0.1 μg/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.
Application
Immunofluorescence of CXCR4 in HeLa cells with CXCR4 antibody at 20 μg/mL.
Application
Western blot analysis of CXCR4 in HeLa whole cell lysate with CXCR4 antibody at 0.5 μg/mL.| Application Notes | CXCR4 antibody can be used for Western blot at 1 - 2 μg/mL dilution and for immunoprecipitation and immunocytochemistry at 10 μg/mL. Immunofluorescence starting at 20 μg/mL. Flow Cytometry at 0.1 μg/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CXCR4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CXCR4 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM276481