Application
Antibody used in IHC on Human Lung.| Product Name | CYBB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYBB, CGD, GP91-1, GP91-PHOX, GP91PHOX, NOX2, AMCBX2, p91-PHOX |
| Product description | CYBB is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H (+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD).Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Fink, K., (2008) J. Immunol. 180 (10), 6911-6922. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CYBB. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CYBB antibody can be used for detection of CYBB by ELISA at 1:1562500. CYBB antibody can be used for detection of CYBB by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYBB |
|---|---|
| Alternative Names | CYBB CGD GP91-1 GP91-PHOX GP91PHOX NOX2 AMCBX2 p91-PHOX |
| Molecular Weight(MW) | 63 kDa |
Application
Antibody used in IHC on Human Lung.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | CYBB antibody can be used for detection of CYBB by ELISA at 1:1562500. CYBB antibody can be used for detection of CYBB by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CYBB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
