Application
Immunohistochemistry of NOX2 in rat brain tissue with NOX2 antibody at 5 μg/ml.Application
Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.Application
Immunohistochemistry of NOX2 in rat brain tissue cells with NOX2 antibody at 2 μg/ml.Application
Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.Application
Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 μg/ml.| Product Name | NOX2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOX2 Antibody: CGD, NOX2, AMCBX2, GP91-1, GP91PHOX, p91-PHOX, GP91-PHOX, Cytochrome b-245 heavy chain, CGD91-phox, Cytochrome b558 subunit beta |
| Product description | The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX2, also known as cytochrome b beta (CYBB) is one of two proteins that make up Cytochrome b-245, thought to be a primary component of the microbicidal oxidase system of phagocytes. NOX2 deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD) (2). Activation of the NOX2 enzyme complex in microglia is thought to be neurotoxic and may play a role in Alzheimer’s and Parkinson’s disease (3).1) Bedard K and Krause KH. The Nox family of ROS-generating NAPDH oxidases: physiology and pathophysiology. Physiol. Rev. 2007; 87:245-313. |
| Immunogen | Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2. The immunogen is located within amino acids 140 - 190 of NOX2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYBB |
|---|---|
| Alternative Names | NOX2 Antibody: CGD NOX2 AMCBX2 GP91-1 GP91PHOX p91-PHOX GP91-PHOX Cytochrome b-245 heavy chain CGD91-phox Cytochrome b558 subunit beta |
| Molecular Weight(MW) | Predicted: 59, 63 kDa Observed: 58 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (87%), Bovine: (87%) |
| Tissue Specificity | NOX2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins. |
Application
Immunohistochemistry of NOX2 in rat brain tissue with NOX2 antibody at 5 μg/ml.
Application
Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.
Application
Immunohistochemistry of NOX2 in rat brain tissue cells with NOX2 antibody at 2 μg/ml.
Application
Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.
Application
Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NOX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | NOX2 antibody is supplied in PBS containing 0.02% sodium azide. |
