Application
Western blot analysis in Hela cell line lysates (35ug/lane).Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CYC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.1) Johnatty, S.E., et al. PLoS Genet.(7), E1001016 (2010) : |
| Immunogen | This CYC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-291 amino acids from the C-terminal region of human CYC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYC1 |
|---|---|
| Alternative Names | Cytochrome c1 heme protein mitochondrial Complex III subunit 4 Complex III subunit IV Cytochrome b-c1 complex subunit 4 Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit Cytochrome c-1 CYC1 |
| Molecular Weight(MW) | 35 kDa |
Application
Western blot analysis in Hela cell line lysates (35ug/lane).
Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
