Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).Application
CYC1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | CYC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.1) Cummings, C., et al. Apoptosis 11(7):1121-1129(2006) Wen, J.J., et al. Free Radic. Biol. Med. 37(12):2072-2081(2004) An, J., et al. J. Biol. Chem. 279(18):19133-19140(2004) |
| Immunogen | This CYC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the C-terminal region of human CYC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYC1 |
|---|---|
| Alternative Names | Cytochrome c1 heme protein mitochondrial Complex III subunit 4 Complex III subunit IV Cytochrome b-c1 complex subunit 4 Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit Cytochrome c-1 CYC1 |
| Molecular Weight(MW) | 35 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).
Application
CYC1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
