Application
Deoxycytidine kinase(DCK) immunoprecipitated from H460cells with 7.5ug (microgram) of the dCK antibody (AP7087b) using the Pierce classic mammalian IP kit (#45217) reagent as described as manufacturer instructions (lane 1, 3) and Current Protocols in Cell Biology, 1998, 7.2.1-7.2.21. Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1:700 dilution of DCK antibodyApplication
Antibody is used in Western blot to detect DCK in mouse intestine tissue lysate.Application
Confocal immunofluorescent analysis of DCK Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
DCK Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | DCK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Deoxycytidine kinase is responsible for the phosphorylation of several deoxyribonucleosides and their analogs. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. It is the rate limiting enzyme in the activation of many important anticancer and retroviral drugs and its activity is often decreased in cells that are resistant to cytosine arabinoside.1) Chottiner, E. G., et al. Proc. Nat. Acad. Sci. 88: 1531-1535 (1991). |
| Immunogen | This DCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the C-terminal region of human DCK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IF,IHC-P,IP,WB |
| For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IP starting dilution is: 1:500~1000 | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | DCK |
|---|---|
| Alternative Names | Deoxycytidine kinase dCK DCK |
| Molecular Weight(MW) | 31 kDa |
Application
Deoxycytidine kinase(DCK) immunoprecipitated from H460cells with 7.5ug (microgram) of the dCK antibody (AP7087b) using the Pierce classic mammalian IP kit (#45217) reagent as described as manufacturer instructions (lane 1, 3) and Current Protocols in Cell Biology, 1998, 7.2.1-7.2.21. Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1:700 dilution of DCK antibody
Application
Antibody is used in Western blot to detect DCK in mouse intestine tissue lysate.
Application
Confocal immunofluorescent analysis of DCK Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
DCK Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IP starting dilution is: 1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
