Application
Immunohistochemical analysis of paraffin - embedded human smooth musde sarcoma showing cytoplasmic location using Desmin antibody with DAB staining.Application
Western blot analysis using Desmin monoclonal antibody against truncated Desmin recombinant protein| Product Name | Desmin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CSM1, CSM2, LGMD2R |
| Product description | Desmin (DES), with 470-amino acid protein (about 52 kDa), belongs to the intermediate filament family and Desmin is class III intermediate filaments found in muscle cells. Homopolymers of Desmin form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane.Mutations in Desmin are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.Desmin is also expressed in smooth muscle cells of both airways and alveolar ducts and Desmin is a load-bearing protein that stiffens the airways and consequently the lung and modulates airway contractile response.1) Felix R. Shardonofsky, Yassemi Capetanaki, and Aladin M. Boriek. Am J Physiol Lung Cell Mol Physiol, May 2006; 290: L890 - L896. |
| Immunogen | Ni-NTA purified truncated recombinant Desmin expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:1,000 - 1:2,000 IHC(P):1:1,000 - 1:2,000 IHC(F):1:1,000 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | DES |
|---|---|
| Alternative Names | CSM1 CSM2 LGMD2R |
Application
Immunohistochemical analysis of paraffin - embedded human smooth musde sarcoma showing cytoplasmic location using Desmin antibody with DAB staining.
Application
Western blot analysis using Desmin monoclonal antibody against truncated Desmin recombinant protein| Application Notes | Western Blot:1:1,000 - 1:2,000 IHC(P):1:1,000 - 1:2,000 IHC(F):1:1,000 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Desmin monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide |
