Application
Western blot analysis of anti-DHCR24 Antibody in HepG2 cell line lysates (35ug/lane)| Product Name | DHCR24 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Delta(24)-sterol reductase, 24-dehydrocholesterol reductase, 3-beta-hydroxysterol delta-24-reductase, Diminuto/dwarf1 homolog, Seladin-1, DHCR24, KIAA0018 |
| Product description | DHCR24 is a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. This protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in DHCR24 gene have been associated with desmosterolosis. Also, reduced expression of DHCR24 gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.1) Greeve I., Hermans-Borgmeyer I.J. Neurosci. 20:7345-7352(2000) |
| Immunogen | This DHCR24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human DHCR24. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | DHCR24 |
|---|---|
| Alternative Names | Delta(24)-sterol reductase 24-dehydrocholesterol reductase 3-beta-hydroxysterol delta-24-reductase Diminuto/dwarf1 homolog Seladin-1 DHCR24 KIAA0018 |
| Molecular Weight(MW) | 60 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of anti-DHCR24 Antibody in HepG2 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
