WB
Western blot analysis of DHCR24 on different lysates with Rabbit anti-DHCR24 antibody at 1/5,000 dilution.Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds;Low expression: MDA-MB-231(PMID: 32713162).Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: Hep G2 (Human liver cancer cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody:1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Brain. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Testis. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.FC
Application: Flow Cytometry (Intra). Species: Human. Sample: Hep G2 (Human liver cancer cells). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: (1/1,000, Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (488), 15 minutes at room temperature.| Product Name | DHCR24 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human DHCR24 aa 53-282. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000 ICC/IF:1:500 IHC:1:200-1:1000 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DHCR24 |
|---|---|
| Gene Synonyms | DCE SELADIN1 Nbla03646 seladin-1 |
| Gene Full Name | 24-dehydrocholesterol reductase |
| Gene Summary | This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq, Jul 2008] |
| Alternative Names | 24 dehydrocholesterol reductase antibody 24-dehydrocholesterol reductase antibody 3 beta hydroxysterol delta 24 reductase antibody 3-beta-hydroxysterol delta-24-reductase antibody DCE antibody Delta(24)-sterol reductase antibody Desmosterol to cholesterol enzyme antibody DHC24_HUMAN antibody Dhcr24 antibody Diminuto dwarf1 homolog antibody 24 dehydrocholesterol reductase antibody 24-dehydrocholesterol reductase antibody 3 beta hydroxysterol delta 24 reductase antibody 3-beta-hydroxysterol delta-24-reductase antibody DCE antibody Delta(24)-sterol reductase antibody Desmosterol to cholesterol enzyme antibody DHC24_HUMAN antibody Dhcr24 antibody Diminuto dwarf1 homolog antibody Diminuto/dwarf1 homolog antibody KIAA0018 antibody Nbla03646 antibody Seladin-1 antibody SELADIN1 antibody Selective AD indicator 1 antibody Selective Alzheimer's Disease Indicator 1 antibody |
| Molecular Weight(MW) | 60 kDa(Observed band size: 54 kDa) |
| Cellular Localization | Endoplasmic reticulum membrane, Golgi apparatus membrane. |
WB
Western blot analysis of DHCR24 on different lysates with Rabbit anti-DHCR24 antibody at 1/5,000 dilution.Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds;Low expression: MDA-MB-231(PMID: 32713162).Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: Hep G2 (Human liver cancer cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody:1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Brain. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Testis. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
FC
Application: Flow Cytometry (Intra). Species: Human. Sample: Hep G2 (Human liver cancer cells). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: (1/1,000, Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (488), 15 minutes at room temperature.| Application Notes | WB:1:1000 ICC/IF:1:500 IHC:1:200-1:1000 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
