Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western Blot at 1:1000 dilution + mouse cerebellum lysate Lysates/proteins at 20 ug per lane.| Product Name | Dnmt2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, MHsaIIP, PuMet, TRDMT1, DNMT2 |
| Product description | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a protein with similarity to DNA methyltransferases, but this protein does not display methyltransferase activity. The protein strongly binds DNA, suggesting that it may mark specific sequences in the genome. Alternative splicing results in multiple transcript variants encoding different isoforms.1) Hermann, A., et al., J. Biol. Chem. 278(34):31717-31721 (2003). |
| Immunogen | This Dnmt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from human Dnmt2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | TRDMT1 |
|---|---|
| Alternative Names | tRNA (cytosine(38)-C(5))-methyltransferase DNA (cytosine-5)-methyltransferase-like protein 2 Dnmt2 DNA methyltransferase homolog HsaIIP DNA MTase homolog HsaIIP MHsaIIP PuMet TRDMT1 DNMT2 |
| Molecular Weight(MW) | 45 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western Blot at 1:1000 dilution + mouse cerebellum lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
