WB
Western blot analysis of Dnmt2 on different lysates with Rabbit anti-Dnmt2 antibody at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate, Lane 2: HeLa cell lysate, Lane 3: 293T cell lysate, Lane 4: HCT 116 cell lysate, Lane 5: U-2 OS cell lysate, Lane 6: COS-1 cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Dnmt2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of MCF7 cells labeling Dnmt2 with Rabbit anti-Dnmt2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dnmt2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.FC
Flow cytometric analysis of MCF7 cells labeling Dnmt2. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Dnmt2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Dnmt2 aa 1-391 / 391. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000 IHC:1:2000 ICC/IF:1:100 FC:1:1000 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TRDMT1 |
|---|---|
| Gene Synonyms | DMNT2 DNMT2 PUMET RNMT1 MHSAIIP |
| Gene Full Name | tRNA aspartic acid methyltransferase 1 |
| Gene Summary | This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010] |
| Alternative Names | dDNMT antibody DmMT 2 antibody DmMT2 antibody DNA (cytosine 5 ) methyltransferase 2 antibody DNA (cytosine 5) methyltransferase like protein 2 antibody DNA (cytosine-5)-methyltransferase-like protein 2 antibody DNA 5 cytosine methyltransferase antibody DNA methyltransferase 2 antibody DNA methyltransferase homolog HsaIIP antibody DNA MTase homolog HsaIIP antibody dDNMT antibody DmMT 2 antibody DmMT2 antibody DNA (cytosine 5 ) methyltransferase 2 antibody DNA (cytosine 5) methyltransferase like protein 2 antibody DNA (cytosine-5)-methyltransferase-like protein 2 antibody DNA 5 cytosine methyltransferase antibody DNA methyltransferase 2 antibody DNA methyltransferase homolog HsaIIP antibody DNA MTase homolog HsaIIP antibody Dnmt 2 antibody Dnmt2 antibody M.HsaIIP antibody MHsaIIP antibody nmt 2 antibody nmt2 antibody OTTHUMP00000045198 antibody PuMet antibody RNMT 1 antibody RNMT1 antibody TRDMT 1 antibody TRDMT_HUMAN antibody TRDMT1 antibody tRNA (cytosine 5 ) methyltransferase antibody tRNA (cytosine(38)-C(5))-methyltransferase antibody tRNA aspartic acid methyltransferase 1 antibody tRNA aspartic acid methyltransferase 1 variant 1 antibody tRNA aspartic acid methyltransferase 1 variant 2 antibody tRNA aspartic acid methyltransferase 1 variant 3 antibody tRNA aspartic acid methyltransferase 1 variant 4 antibody tRNA aspartic acid methyltransferase 1 variant 5 antibody tRNA aspartic acid methyltransferase 1 variant 8 antibody |
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of Dnmt2 on different lysates with Rabbit anti-Dnmt2 antibody at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate, Lane 2: HeLa cell lysate, Lane 3: 293T cell lysate, Lane 4: HCT 116 cell lysate, Lane 5: U-2 OS cell lysate, Lane 6: COS-1 cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Dnmt2 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of MCF7 cells labeling Dnmt2 with Rabbit anti-Dnmt2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dnmt2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
FC
Flow cytometric analysis of MCF7 cells labeling Dnmt2. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 IHC:1:2000 ICC/IF:1:100 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
