Application
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with DOLK Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of DOLK Antibody in 293 cell line lysates (35ug/lane)| Product Name | DOLK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dolichol kinase, Transmembrane protein 15, DOLK, KIAA1094, TMEM15 |
| Product description | DOLK is involved in the synthesis of the sugar donor Dol-P-Man which is required in the synthesis of N-linked and O-linked oligosaccharides and for that of GPI anchors (By similarity).1) Shridas,P. et.al., J. Biol. Chem. 281 (42), 31696-31704 (2006) |
| Immunogen | This DOLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-339 amino acids from the Central region of human DOLK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DOLK |
|---|---|
| Alternative Names | Dolichol kinase Transmembrane protein 15 DOLK KIAA1094 TMEM15 |
| Molecular Weight(MW) | 59 kDa |
Application
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with DOLK Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of DOLK Antibody in 293 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
