Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Product Name | DPH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DPH2, DPH2L2 |
| Product description | DPH2 gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.1) Liu, S., (2004) Mol. Cell. Biol. 24 (21), 9487-9497. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DPH2 antibody can be used for detection of DPH2 by ELISA at 1:62500. DPH2 antibody can be used for detection of DPH2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DPH2 |
|---|---|
| Alternative Names | DPH2 DPH2L2 |
| Molecular Weight(MW) | 52 kDa |
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | DPH2 antibody can be used for detection of DPH2 by ELISA at 1:62500. DPH2 antibody can be used for detection of DPH2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DPH2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
