Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Product Name | DPH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DPH2, DPH2L2 |
| Product description | This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.1) Liu, S., (2004) Mol. Cell. Biol. 24 (21), 9487-9497. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DPH2 antibody can be used for detection of DPH2 by ELISA at 1:1562500. DPH2 antibody can be used for detection of DPH2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DPH2 |
|---|---|
| Alternative Names | DPH2 DPH2L2 |
| Molecular Weight(MW) | 52 kDa |
Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | DPH2 antibody can be used for detection of DPH2 by ELISA at 1:1562500. DPH2 antibody can be used for detection of DPH2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DPH2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
