Application
Immunocytochemistry of DR5 in HeLa cells with DR5 antibody at 5 μg/mL.Application
Immunofluorescence of DR5 in HeLa cells with DR5 antibody at 20 μg/mL.Application
Western blot analysis of DR5 in HeLa (H) and K562 (K) cell lysates with DR5 antibody at 2 μg/mL.| Product Name | DR5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DR5 Antibody: DR5, CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL-R2, KILLER/DR5, DR5, UNQ160/PRO186, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TRAIL receptor 2 |
| Product description | DR5 Antibody: Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently. Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-κB.1) Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 1997;277:815-8 |
| Immunogen | DR5 antibody was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human DR5 precursor. The immunogen is located within the last 50 amino acids of DR5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| DR5 antibody can be used for detection of DR5 expression by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF10B |
|---|---|
| Alternative Names | DR5 Antibody: DR5 CD262 KILLER TRICK2 TRICKB ZTNFR9 TRAILR2 TRICK2A TRICK2B TRAIL-R2 KILLER/DR5 DR5 UNQ160/PRO186 Tumor necrosis factor receptor superfamily member 10B Death receptor 5 TRAIL receptor 2 |
| Sequence Similarities | 3) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | Antibody has no cross reaction to DR4. |
Application
Immunocytochemistry of DR5 in HeLa cells with DR5 antibody at 5 μg/mL.
Application
Immunofluorescence of DR5 in HeLa cells with DR5 antibody at 20 μg/mL.
Application
Western blot analysis of DR5 in HeLa (H) and K562 (K) cell lysates with DR5 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | DR5 antibody can be used for detection of DR5 expression by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DR5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DR5 Antibody is supplied in PBS containing 0.02% sodium azide. |
