Application
Flow cytometric analysis of MCF-7 cells using (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of anti (AP7795a) in T47D cell line lysates (35ug/lane)| Product Name | DSG2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Desmoglein-2, Cadherin family member 5, HDGC, DSG2, CDHF5 |
| Product description | Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This protein is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in the DSG2 gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10.1) Posch,M.G., Mol. Genet. Metab. 95 (1-2), 74-80 (2008) |
| Immunogen | This DSG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-216 amino acids from the N-terminal region of human DSG2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | DSG2 |
|---|---|
| Alternative Names | Desmoglein-2 Cadherin family member 5 HDGC DSG2 CDHF5 |
| Molecular Weight(MW) | 122 kDa |
Application
Flow cytometric analysis of MCF-7 cells using (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of anti (AP7795a) in T47D cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
