Application
Western blot analysis in U251 cell line lysates (35ug/lane).Application
Western blot analysis in mouse testis tissue lysates (35ug/lane).Application
EIF2B5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | EIF2B5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Translation initiation factor eIF-2B subunit epsilon, eIF-2B GDP-GTP exchange factor subunit epsilon, EIF2B5, EIF2BE |
| Product description | This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.1) van der Lei, H.D., et al. Neurology 75(17):1555-1559(2010) |
| Immunogen | This EIF2B5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the N-terminal region of human EIF2B5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2B5 |
|---|---|
| Alternative Names | Translation initiation factor eIF-2B subunit epsilon eIF-2B GDP-GTP exchange factor subunit epsilon EIF2B5 EIF2BE |
| Molecular Weight(MW) | 80 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in U251 cell line lysates (35ug/lane).
Application
Western blot analysis in mouse testis tissue lysates (35ug/lane).
Application
EIF2B5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
