WB
Western blot analysis of EIF2B5 on different lysates with Rabbit anti-EIF2B5 antibody at 1/1000 dilution. Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: Jurkat cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling EIF2B5 with Rabbit anti-EIF2B5 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EIF2B5 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1000 dilution.| Product Name | EIF2B5 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human EIF2B5 aa 1-50 / 721. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 IHC:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2B5 |
|---|---|
| Gene Synonyms | CLE CACH LVWM VWM5 EIF-2B EIF2Bepsilon |
| Gene Full Name | eukaryotic translation initiation factor 2B subunit epsilon |
| Gene Summary | This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Nov 2009] |
| Alternative Names | CACH antibody CLE antibody EI2BE_HUMAN antibody EIF 2B antibody eIF 2B GDP GTP exchange factor antibody eIF 2B GDP GTP exchange factor subunit epsilon antibody eIF-2B GDP-GTP exchange factor subunit epsilon antibody EIF2B antibody EIF2B5 antibody EIF2BE antibody CACH antibody CLE antibody EI2BE_HUMAN antibody EIF 2B antibody eIF 2B GDP GTP exchange factor antibody eIF 2B GDP GTP exchange factor subunit epsilon antibody eIF-2B GDP-GTP exchange factor subunit epsilon antibody EIF2B antibody EIF2B5 antibody EIF2BE antibody EIF2Bepsilon antibody Eukaryotic Translation Initiation Factor 2 Beta Epsilon antibody Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa antibody Eukaryotic translation initiation factor 2B subunit 5 epsilon antibody LVWM antibody Translation initiation factor eIF 2B subunit epsilon antibody Translation initiation factor eIF-2B subunit epsilon antibody |
| Molecular Weight(MW) | 80kDa(Observed band size:80kDa) |
| Cellular Localization | Cytoplasm, cytosol. |
WB
Western blot analysis of EIF2B5 on different lysates with Rabbit anti-EIF2B5 antibody at 1/1000 dilution. Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: Jurkat cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling EIF2B5 with Rabbit anti-EIF2B5 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EIF2B5 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1000 dilution.| Application Notes | WB:1:1000 IHC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
