Application
Immunofluorescence of EMX2 in human lung tissue with EMX2 antibody at 20 μg/mL.Application
Immunohistochemistry of EMX2 in human lung tissue with EMX2 antibody at 2.5 μg/mL.Application
Western blot analysis of EMX2 in human lung tissue lysate with EMX2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | EMX2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EMX2 Antibody: Homeobox protein EMX2, Empty spiracles homolog 2 |
| Product description | EMX2 Antibody: EMX2, like its closely related homolog EMX1, is a homeobox transcription factor involved in specifying cell fates in the developing central nervous system and participates in the development of olfactory neurons. EMX2 is expressed in the dorsal telencephalon during development in a low rostral-lateral to high caudal-medial gradient and is proposed to pattern the neocortex into defined functional areas. It is also expressed in embryonic and adult olfactory neuroepithelia where it complexes with eukaryotic translation initiation factor 4E (eIF4E) and possibly regulates mRNA transport or translation. In the developing urogenital system, it is expressed in epithelial tissues and is negatively regulated by HOXA10.1) Bishop KM, Garel S, Nakagawa Y, et al. EMX1 and Emx2 cooperate to regulate cortical size, lamination, neuronal differentiation, development of cortical efferents, and thalamocortical pathfinding. J. Comp. Neurol. 2003; 457:345-60. |
| Immunogen | EMX2 antibody was raised against an 18 amino acid synthetic peptide near the center of human EMX2. The immunogen is located within amino acids 60 - 110 of EMX2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| EMX2 antibody can be used for detection of EMX2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EMX2 |
|---|---|
| Alternative Names | EMX2 Antibody: Homeobox protein EMX2 Empty spiracles homolog 2 |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | At least two isoforms of EMX2 are known to exist; this antibody will detect both isoforms. EMX2 antibody is predicted to not cross-react with EMX1 |
Application
Immunofluorescence of EMX2 in human lung tissue with EMX2 antibody at 20 μg/mL.
Application
Immunohistochemistry of EMX2 in human lung tissue with EMX2 antibody at 2.5 μg/mL.
Application
Western blot analysis of EMX2 in human lung tissue lysate with EMX2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | EMX2 antibody can be used for detection of EMX2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EMX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | EMX2 Antibody is supplied in PBS containing 0.02% sodium azide. |
