The homeodomain transcription factor EMX2 is critical for central nervous system and urogenital development. EMX1 along with EMX2 is related to the 'empty spiracles' gene expressed in the developing Drosophila head.1) Daftary, G.S. et al., (2004) J. Clin. Endocrinol. Metab. 89 (5), 2390-2396.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EMX2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
EMX2 antibody can be used for detection of EMX2 by ELISA at 1:312500. EMX2 antibody can be used for detection of EMX2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Muscle at 4.0-8.0 ug/ml.
Application
Antibody used in IHC on Human Brain, cerebellum at 5 ug/ml.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
EMX2 antibody can be used for detection of EMX2 by ELISA at 1:312500. EMX2 antibody can be used for detection of EMX2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EMX2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.