Application
Immunocytochemistry of Eotaxin in 293 cells with Eotaxin antibody at 5 μg/mL.Application
Immunofluorescence of Eotaxin in 293 cells with Eotaxin antibody at 10 μg/mL.Application
Western blot analysis of eotaxin in HeLa cell lysate containing 10, 2.5, or 1 ng of full length recombinant eotaxin with eotaxin antibody at 1 μg/mL.| Product Name | Eotaxin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Eotaxin Antibody: SCYA11, SCYA11, Eotaxin, C-C motif chemokine 11 |
| Product description | Eotaxin Antibody: Chemokines play a key role in inflammation. The CC chemokine eotaxin is a potent and specific eosinophil chemoattractant that is expressed by a variety of cell types in certain inflammatory conditions. Some G-protein coupled chemokine receptors are also utilized as virus coreceptors for fusion and infection of cells. The eotaxin receptor CCR3 is required for HIV-1 entry into target cells such as microglia and eotaxin inhibits the infection of HIV-1.1) Kitaura M, Nakajima T, Imai T, et al. Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3. J Biol Chem 1996;271:7725-30 |
| Immunogen | Eotaxin antibody was raised in rabbits against a peptide corresponding to amino acids near the carboxy terminus of human eotaxin. The immunogen is located within the last 50 amino acids of Eotaxin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Eotaxin antibody can be used for detection of eotaxin by Western blot at 1 μg/mL. The recombinant protein can be used as positive control and monomer and homodimer are detected at approximate 9 and 18 kDa. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCL11 |
|---|---|
| Alternative Names | Eotaxin Antibody: SCYA11 SCYA11 Eotaxin C-C motif chemokine 11 |
| Molecular Weight(MW) | 9 - 18 kDa |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
Application
Immunocytochemistry of Eotaxin in 293 cells with Eotaxin antibody at 5 μg/mL.
Application
Immunofluorescence of Eotaxin in 293 cells with Eotaxin antibody at 10 μg/mL.
Application
Western blot analysis of eotaxin in HeLa cell lysate containing 10, 2.5, or 1 ng of full length recombinant eotaxin with eotaxin antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | Eotaxin antibody can be used for detection of eotaxin by Western blot at 1 μg/mL. The recombinant protein can be used as positive control and monomer and homodimer are detected at approximate 9 and 18 kDa. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Eotaxin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Eotaxin Antibody is supplied in PBS containing 0.02% sodium azide. |
